Coding

Part:BBa_J56003:Design

Designed by: Matt Eames   Group: iGEM06_UCSF   (2006-10-13)


Tar mutated to respond to phenylalanine instead of aspartate


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1660
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1660
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1660
    Illegal NgoMIV site found at 829
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

Engineered receptor mutated from Tar to respond to phenylalanine. Interacts with wild-type CheW. Includes a XbaI restriction site at terminus.

Source

Synthesized by DNA 2.0

References

Derr et al, JMB (2006)